ACE and vibrio infectious disease: cholerae strains from water samples collected at epidemic sites of a 2005 cholera outbreak occurred in the Nansha District of Guangzhou in China, and found that all the isolates were negative for either both ctxA and tcpA or all the four genes ctxA, tcpA, ace, and zot, except for one strain that was positive for all four genes (Li et al. 2015).