Nonetheless, given the feasibility of detecting 2HG in the clinic, and considering our findings that a drop in 2HG is associated with response to PI3K inhibition, this metabolite could serve as an indicator of drug target modulation in IDHmut glioma, providing an MRS detectable noninvasive imaging biomarker of response to PI3K/mTOR inhibitors that is independent of tumor size. This evidence concerns the gene MTOR and central nervous system cancer.