When comparing differences in CD206 expression between OVCAR3/M0 or CSC/M0 hetero-spheroids, a significant (**p < 0.001, one-way ANOVA) 20% increase in CD206 was observed with CSC co-culture (Fig. 2a), indicating that ovarian CSCs drive an immuno-suppressive phenotype in macrophages compared to bulk ovarian cancer cells. This evidence concerns the gene MRC1 and ovarian carcinoma.