Nevertheless, the earlier studies had various limitations: lack of precise examination of steatosis and fibrosis of liver by an accurate none-invasive method such as transient elastography (Fibroscan) [37, 42, 43], low study power [42], lack of assessing other important factors such as adipokines, adiponectin, as well as inflammatory and oxidative stress markers [37, 42, 43]. This evidence concerns the gene ADIPOQ and steatosis.