The two main differences between the two works are (1) the melanoma data used by Schelker et al. only provided sufficient information for distinguish nine major cell types and three T cell subtypes, whereas the HNSCC data we studied was able to further separate exhaustive CD8 T cells and provide corresponding reference GEP; (2) In our method, we used both marker gene information and a global ssGSEA scores to determine cell types from adaptive clustering analysis. This evidence concerns the gene CD8A and head and neck squamous cell carcinoma.