Taking advantage of an epigenetic unmasking approach used to reverse genomic hypermethylation marks, we highlighted a set of six miRNAs epigenetically silenced in hepatocellular carcinoma (HCC) cells and located in the imprinted delta like non-canonical Notch ligand 1 (DLK1)-maternally expressed 3 (MEG3) locus, in which miR-493-5p repression was correlated with MEG3-differentially regulated region (DMR) methylation. Here, DLK1 is linked to hepatocellular carcinoma.