IGF2 and hepatocellular carcinoma: Among 278 predicted targets, 2 genes appeared downregulated in our microarray data (Fig. 5c): hematological and neurological expressed 1 (HN1) and IGF2. We also performed Gene Set Enrichment Analysis (GSEA) and observed relevant enrichment of three sets of genes in HCC cells overexpressing miR-493-5p, which was consistent with the antitumor effect of the microRNA (Supplementary Fig. 8).