Here, we investigated the role of MEK pathway in regulating IFN response in human airway epithelial cells and we show that pharmacological inhibition of MEK enhances type I IFN response through improved translation control of IRF3-driven innate immunity and reduction of the IFNB1 gene repressor, positive regulatory domain I-binding factor1 (PRDI-BF1) but only with RSVA2 infection and not with RV2. The gene discussed is IFNB1; the disease is infection.