Subsequent validation in an independent patient cohort (validation TMA; 18 samples of normal mucosa, 28 euploid CRCs, and 25 aneuploid CRCs) confirmed the increased UBE2N expression in euploid compared to aneuploid CRCs (median 0.3122, 95%CI 0.2687–0.3950 vs. median 0.1973, 95%CI 0.1769–0.2785, p = 0.0070) and in normal mucosa compared to tumor tissue (median 0.3637, 95%CI 0.2776–0.3949 vs. median 0.2562, 95%CI 0.2405–0.3250, p = 0.0374, Fig. 1c). Here, UBE2N is linked to neoplasm.