EGFR and large cell carcinoma: We further used the SRB assay to examine the cytotoxic effect of WA in NSCLC cells, such as A549, CL141 and H441 (adenocarcinoma with wild-type EGFR), CL97 and H1975 (adenocarcinoma harboring EGFR T790M mutation), and CL152 (SCC) and H1299 (large cell carcinoma).