EBI3 and diffuse large B-cell lymphoma: Surprisingly, when we tested anti-IL-35/EBI3 15k8D10 mAb by western blotting against various recombinant proteins or cell lysate, in parallel to a previously characterized specific mouse anti-EBI3 mAb [2G4H6 clone, (17)], it failed to detect recombinant IL-35 produced as a non-covalently linked heterodimer in 293 cells (Figure 2A, lane 4) or EBI3 and p35 subunits individually (Figure 2A, lanes 2, 3), and yielded several non-specific cross-reacting bands in the cell lysate from OCI-Ly3 DLBCL cell line (Figure 2A, lane 1).