SREBF1 and melanoma: To elucidate the molecular mechanism of SREBP1-governed DNFA gene activation in melanoma cells, we used a chromatin immunoprecipitation (ChIP)-qPCR assay to detect occupancy of SREBP1, RNA polymerase II (RNAP II), and H3K36me3 – a histone marker associated with transcription elongation60 on DNFA genes.