We found an increase in late apoptotic events, which ranged from 20 to 30% after treatment with oleacein 5.0 and 10.0 μM, respectively (Figure 2C); the increase in cleaved PARP1, caspase-3 and caspase-8 on oleacein-treated MM cell lines, as shown by Western Blot (WB), further confirmed apoptosis induction (Figure 2D); no activation of caspase-7 and -9 was observed (Supplementary Figure S1), thus indicating that oleacein predominantly activates the extrinsic apoptotic pathway. This evidence concerns the gene PARP1 and Miyoshi myopathy.