Given the similarity between zebrafish MAPK pathways and those of mammals [37, 38], to evaluate the function of Trxlp in the regulation of ASK1-MAPK signaling in vivo, we developed a microinjection infection model [38] using 3 days post fertilized (dpf) zebrafish larvae for analyzing the pathogenesis of E. piscicida (Fig 5A). Here, MAP3K5 is linked to infection.