Western Blot (WB) results (Fig. 10) indicated that NE infection resulted in a significant increase in the relative levels of MLCK protein and a reduction in ZO-1 protein levels in the jejunum mucosa compared with the non-challenged groups but no significant difference was observed in the abundance of CLDN-1, CLDN-3, and OCLN protein between the infected and non-infected groups. The gene discussed is CLDN1; the disease is infection.