We confirmed that these tumor-derived cells were indeed KGN/RUNX3 cells by detecting expression of RUNX3-FLAG, as determined by immunoblotting, using a specific RUNX3 antibody or an anti-FLAG antibody (Figure 3B), as well as by quantitative reverse transcription-PCR (qRT-PCR) using primers specific to human RUNX3 (data not shown). Here, RUNX3 is linked to neoplasm.