To determine whether RUNX3 promotes KGN tumor formation in a mouse xenograft model, we subcutaneously injected KGN/Vector and KGN/RUNX3 cells into contralateral flanks of female NSG (NOD-scid IL2R-gammanull) mice and monitored tumor formation twice a week by palpation for up to 185 days. Here, RUNX3 is linked to neoplasm.