To investigate the role of HCG11 in tumorigenesis of glioma, we designed and performed gain‐of‐function assays in two glioma cells which possessed the lowest expression level of HCG11. Before the functional assays, HCG11 was overexpressed in U87 and U251 cells by transfecting with pcDNA/HCG11. Here, HCG11 is linked to central nervous system cancer.