MAP1LC3B and neurodegenerative disease: Since impaired autophagy has been implicated in the pathogenesis of neurodegeneration and age is the most relevant risk factor for neurodegenerative disease, we used the GFP-LC3B probe to assess how biogenesis rates change with age in primary DRG neurons dissected from mice of four different ages: 1-month-old young mice, 3-month-old young adult mice, 16–17 month-old aged mice, and 24-month-old advanced aged mice.