Combined treatment of HCC cells with allicin and 5-fluorouracil (5-FU) increased intracellular ROS level, activated caspase 3, and PARP reduced Bcl-2 and decreased the mitochondrial membrane potential, compared with DMSO, 5-FU, and allicin treated alone, which suggest that allicin enhanced HCC cells to 5-FU induced apoptosis via ROS-mediated mitochondrial pathway [54]. Here, PARP1 is linked to hepatocellular carcinoma.