To clarify whether accumulation of AF-AGEs occurs in hepatocytes treated with 1,5-AF, HepG2 cells were incubated with 0–25 mM 1,5-AF and cell extracts were subjected to slot blotting (SB) with the immunopurified anti-AF-AGE antibody to detect intracellular AF-AGEs. The gene discussed is RENBP; the disease is atrial fibrillation.