Finally, four miRNAs (rno-miR-126a-5p, rno-miR-322-5p, rno-miR-30c-5p, and rno-miR-378b) and 10 genes (Gstm1, Gpnmb, Ephx1, Arntl, Cstb, Cndp1, Ppp1r1b, Ppp1r1a, Gng7, and Irf2bp1 (interferon regulatory factor 2 binding protein 1)) that were demonstrated to be aberrantly expressed in CI-AKI showed RT-qPCR results that were in accordance with the sequencing results (Fig 6A and 6B, S7 Table). The gene discussed is PPP1R1B; the disease is acute kidney injury.