IFN-γ is primarily a Th1 cell cytokine and present in human skin in many skin inflammatory disorders and impact keratinocyte proliferation, apoptosis, migration, etc.16,17 To examine if HPKs obtained from hydrogel are functionally responsive to immune effector molecules such as IFN-γ, HPKs expanded on 10 kPa and 20 kPa gel and control at early and late passages were cultured in presence of IFN-γ and surface expression of HLA DR was examined by staining with HLA DR specific mAb and were analyzed by flowcytometry. The gene discussed is IFNG; the disease is inflammatory skin disease.