To further confirm the role of HMGB1 in the senescence of MSCs from SLE patients, different concentrations of HMGB1(1,10,100,500ng/ml) were used to stimulate BM-MSCs, which resulted in the activation of TLR4/NF-κB signaling pathway and the senescence of BM-MSCs. The gene discussed is TLR4; the disease is systemic lupus erythematosus.