Our results showed that HMGB1 could activate the expression of TLR4/NF-κB signaling and promoted cellular senescence in normal BM-MSCs in vitro, which further implied that the TLR4/NF-κB signaling pathway may play an essential role in the senescence of SLE BM-MSCs. This evidence concerns the gene HMGB1 and systemic lupus erythematosus.