Drosophila expressing a PINK1 LoF mutation or the PD-associated mutation G2019S in LRRK2 displayed impaired calcium homeostasis at ER–mitochondrial contact sites, which resulted in mitochondrial dysfunction and loss of dopaminergic neurons, and Miro1 was identified as key player in this process (25). This evidence concerns the gene RHOT1 and Parkinson disease.