Although not significant, there was a trend of reduced AKT2 mRNA expression in 4-week-old offspring (P = 0.056), and reduced IRS2 mRNA expression in 12-week-old offspring (P = 0.052), which was associated with maternal obesity exposure during lactation (Supplementary Table S4). The gene discussed is AKT2; the disease is obesity due to melanocortin 4 receptor deficiency.