Because of the significant sequence identity between cZP1-N1 and its mouse and human counterparts (53% and 51%, respectively; Supplementary Fig. 1a), the crystallographic information reported in this paper provides a solid framework for understanding the effect of the aforementioned mutations on human ZP cross-linking, as well as interpreting additional infertility-associated hZP1-N1 variants that will be reported in the future. The gene discussed is GJA8; the disease is Infertility.