EFNA5 and amyotrophic lateral sclerosis: To determine cell type specific expression of efnA5 and potential single-cell changes related to ALS, we performed RNAscope in situ hybridizations in lumbar spinal cord slices of SOD1WT and SOD1G93A symptomatic mice at 135 days of age and examined EfnA5 gene expression in the ventral horn in neurons and in glial cells (Fig. 2a).