The tumor cells were treated with 40 μg/mL p53 protein for 48 h, and the inhibitory rates of the recombinant protein on MCF-7, MDA-MB-231, and Hep-3B cells reached 52.16, 38.03, and 31.89%, respectively (Fig. 2b and Additional file 1: Figure S4). The gene discussed is TP53; the disease is neoplasm.