Although there is no direct evidence showing that the Tum 5-p53 fusion protein is successfully cleaved by MMPs in the tumor region, the excellent antitumor activity of EcN (Tum 5-p53) indicated that the antitumor efficacy of Tum 5-p53 bifunctional protein does indeed perform significantly better than the p53 protein or Tum-5 protein alone. The gene discussed is TP53; the disease is neoplasm.