The tumor-bearing mice were randomly divided into five groups, and each group was intravenously injected with EcN, EcN (Tum-5), EcN (p53), and EcN (Tum 5-p53) at a dose of 5 × 106 CFUs/100 μL or sterile PBS for animal experiment. The gene discussed is TP53; the disease is neoplasm.