LncRNAs LET, GCASPC and MEG3 were downregulated in gallbladder cancer tissues and acted as cancer suppressors, while the remaining 12 lncRNAs (LINC00152, ANRIL, MALAT1, MINCR, AFAP1-AS1, UCA1, PAGBC, Loc344887, ROR, HEGBC, H19, PVT1), were upregulated in cancer tissues and promoted gallbladder cancer progression. The gene discussed is AFAP1; the disease is gallbladder cancer.