In order to verify whether KHDRBS3, cDENND4C, and miR-577 in GECs might become the potential targets to increase the BTB permeability and improve the DOX across BTB to better induce the apoptosis of U87 glioma cells, the apoptosis rates of U87 cells in the following groups were detected, respectively. Here, KHDRBS3 is linked to central nervous system cancer.