To determine if IL-25 promotes HCC development, and if so by what mechanism, we treated HCC cells (MHCC97L cells and HepG2 cells) with recombinant human IL-25, and found that IL-25 did not remarkably promote the growth and migration of HCC cells (Fig. 2a, Additional file 1: Figure S1A, C and D). The gene discussed is IL25; the disease is hepatocellular carcinoma.