MAPK8 and cyst: Reproduction-mediated JNK signaling hyperactivation is not solely induced by accelerated aging, as puc-lacZ levels in the cyst cells of unmated males cultured at 29°C to hasten aging [50, 51] were much lower than those of mated males cultured at 25°C (Fig 5H), even though the adult survival rates were comparable (S3H Fig).