When egr was overexpressed by tara-Gal4 in unmated males, Zfh-1 expression was induced in Eya-positive cyst cells, a phenotype similar to what we observed in the testes of mated males exhibiting level-1 ectopic Zfh-1 expression (Fig 7P and 7P’, 2N, and column 3 in Fig 2M). This evidence concerns the gene TRIOBP and cyst.