Together, the results presented in Figure 4 and Figure S2 suggest that (a) TcG2/TcG4 DNA vaccine induced Mφ depot was capable of responding to T. cruzi infection by increased proliferation, and proinflammatory activation (antigen uptake, processing, and presentation to CD4+ T cells; production of IL-1β+ and TNF-α cytokines), and (b) fTr based booster immunization had minimal-to-none effects in further enhancing the Mφ response induced by DNA vaccine in mice before or after challenge infection. This evidence concerns the gene TNF and infection.