Further, the frequencies of CD8+T cells responding to challenge infection with a polyfunctional, cytolytic activation (Perforin+CD107+: 24–26.5%, Perforin+TNF-α+: 4.0–5.8%; CD95+CD95L+: 3.6–6.6%) were significantly increased in vaccinated/infected (vs. non-vaccinated/infected) mice (all, *p < 0.05, Figures 6E–G). This evidence concerns the gene PRF1 and infection.