The flow cytometry data showed that vaccinated (vs. non-vaccinated) mice responded to challenge infection with >8-fold increase in the frequency of Th1 cytokine producing (IFN-γ: 3.9–5.1%, TNF-α: 3.5–5.2%) CD8+T cells (all, *p < 0.05, Figure 6F). The gene discussed is TNF; the disease is infection.