Immunophenotypic pluripotency markers profile (CD133, CD90, SSEA-3 and CXCR4) was characterized and set an EPN experimental model, through intracerebroventricular infusion of EPN cells, which was able to replicate the histopathological characteristics of the original tumor with preservation of phenotypic features, of patient tumor, containing pseudorosettes, a histological hallmark of EPN. This evidence concerns the gene PROM1 and neoplasm.