STAT3 and gastric cancer: To determine if TFF1 loss mediates activation of STAT3, we treated organoids derived from TFF1-KO mice with conditioned media from AGS gastric cancer cell lines expressing TFF1 or recombinant TFF1 (400 ng mL−1) for 24 h, and compared them to TFF1-KO organoids treated with condition media from AGS cells expressing empty vector (control).