To determine whether erbB4 promotes the proliferation and survival of human MPNST cells, we targeted ERBB4 expression using shRNAs in cell lines (S462, T265-2c, and MPNST642) with increased erbB4 mRNA and protein expression compared to Schwann cells and assessed total cell number over a 5-day period of growth (Fig. 2a and b). The gene discussed is ERBB4; the disease is malignant peripheral nerve sheath tumor.