CHMP2A and breast cancer: To detect the presence of chimeric BC–ROI combinations in these PCR products, we designed the BC1, BC2, ROI1 and ROI2 primers, specific for the BCs and ROIs of plasmid#1 and plasmid#2 (Fig. 1c) and used BC1/ROI2 and BC2/ROI1 pairs in a test PCR.