To test the impact of AMH mutants on immortalized GnRH neurons’ cell motility and to determine whether AMH promotes such response through an autocrine mechanism, we performed transwell migration assays on GN11 cells either treated with lipofectamine (mock), or transfected with the AMH WT or the AMH variants identified in CHH patients (Figure 7b). The gene discussed is GNRH1; the disease is cartilage-hair hypoplasia.