Our study revealed the increasing trend of the spleen percentage of CD4+ T cells and CD8+ T cells, the Th1/Th2 ratio, the killing activity of CTLs after cryoablation on days 7 and 14 (P < 0.05), coupled with an increase of the spleen NK cell percentage in Group C (Cryoablation group), which suggested that cryoablation treatment can induce an antitumor immune response, which is mainly composed of CD8+ T cells, thereby inhibiting the growth of the untreated tumor, decreasing the rate of lung metastasis, and offering a survival benefit. This evidence concerns the gene CD8A and neoplasm.