Correspondingly, the NKG2D-BBz CAR-T cells efficiently lysed glioblastoma cells and cancer stem cells in vitro and produced high levels of cytokines, perforin and granzyme B. In vivo, the CAR-T cells markedly eliminated xenograft tumors and did not exhibit significant treatment-related toxicity in the treated mice. Here, GZMB is linked to glioblastoma.