However, there are still many challenges that prevent the further applications of CAR-T in glioblastoma, including inhibition of immune cells by glioblastoma microenvironment, lack of general surface antigen due to high glioblastoma heterogeneity, and limited survival time of CAR-T cells within glioblastomas.NKG2D-expressing CARs have many advantages compared to the existing antigens used in glioblastoma CAR-T cell therapy. The gene discussed is KLRK1; the disease is glioblastoma.