For example, in the study of T-ALL by Kishton et al. [173] (§5.2), transformation was generated in vitro by forced expression of an oncogenic mutant of Notch1, and the T-ALL cells were then transferred to irradiated recipient mice and disease allowed to become established prior to AMPK being knocked out by treatment with tamoxifen. The gene discussed is PRKAA2; the disease is acute lymphoblastic leukemia.