For example, in the study of T-ALL by Kishton et al. [173] (§5.2), transformation was generated in vitro by forced expression of an oncogenic mutant of Notch1, and the T-ALL cells were then transferred to irradiated recipient mice and disease allowed to become established prior to AMPK being knocked out by treatment with tamoxifen. Here, PRKAA1 is linked to acute lymphoblastic leukemia.