Given that we identified trpa1, trpv2, trpc3 and trpc6 as ‘prostate-associated’ genes that are deregulated in PTEC compared to that in normal HPrMEC, we further studied their expression at the protein level in vivo by immunohistochemistry on PCa tissues (Figure 2a,b) from ten patients (see materials and methods section) and in vitro by western blotting on proteins extracted from PTEC1-3 (Figure S2). Here, TRPA1 is linked to posterior cortical atrophy.