We therefore applied the custom library to mesenchymal-derived HT1080 fibrosarcoma cells (wild-type TP53, driven by activated NRAS (Q61K) and IDH1 mutation (R132C)) and compared the results to those from ectodermal A375 melanoma cells above (Figure 7, Figure 7—figure supplement 1F & G, Supplementary files 1 & 2). The gene discussed is TP53; the disease is melanoma.