In contrast to the GDM and NC groups, the miR‐351 mimic, siRNA‐FLOT2 and miR‐351 inhibitor + siRNA‐FLOT2 groups showed reduced levels of PI3K, AKT and FLOT2 together with decreased extent of PI3K and AKT phosphorylation (all P < 0.05), while an opposite trend was revealed in the miR‐351 inhibitor group (P < 0.05). This evidence concerns the gene FLOT2 and gestational diabetes.