In conclusion, this study demonstrated that ESVR contains flavonoid derivatives of catechins, anthraquinones, and piceatannol among its chemical constituents and promotes B16F10-Nex2 melanoma cell death via apoptosis induced by caspase-3 activation, the elevation of intracellular calcium and ROS levels, and cell cycle arrest at the G0/G1 phase. The gene discussed is CASP3; the disease is melanoma.