We found that SIRT7 can attenuate LPS-induced upregulation of TLR4 and TAB1 and can also inhibit TAK1 phosphorylation, indicating that SIRT7 is involved in the TLR4-TAK1-TAB1-mediated NF-κB signaling pathway during cattle mastitis occurrence. The gene discussed is SIRT7; the disease is mastitis.