The present study aimed to determine whether blockade of LTCC attenuates DOX-induced cardiomyocyte apoptosis by suppressing intracellular Ca2+ levels and activities of CaMKII and NF-κB and whether it could ameliorate DOX-induced cardiomyopathy by using a low-dose DOX-treated model. This evidence concerns the gene NFKB1 and cardiomyopathy.