Moreover, ZNF423 protein levels and the oxidative stress marker (ox-A1AT) levels were increased in the H2O2 treatment as shown in Figure 2B. ox-A1AT can be used as an acute and chronic oxidative stress marker in CCA and the ox-A1AT levels were correlated with CCA pathogenesis [27,28]. This evidence concerns the gene ZNF423 and cholangiocarcinoma.