We next aimed to identify the mechanism(s) of increased 2-AG and related monoacylglycerol levels (see Table 1) in WD mice by analyzing activity of their biosynthetic [diacylglycerol lipase (DGL)] and degradative enzymes [monoacylglycerol lipase (MGL)] using our lab’s UPLC/MS/MS-based functional enzyme assay methods (see Supplementary Figure S6 for validation of enzyme assays). Here, LIPA is linked to Wilson disease.