However, in LNCaP cells, which expressed lower/undetectable levels of endogenous PTK6 compared with PC3 and MDA-PCa-2b cells (Fig. 5A right upper panel), overexpressing miR-214 alone or miR-214 and PTK6 did not significantly inhibit cell survival, but decreased cell colony formation (Fig. 5B,C, right panels). This evidence concerns the gene PTK6 and posterior cortical atrophy.