PAX6 and infection: We first confirmed the optimal transfection efficiency of the adenoviral plasmid construct with CMV promoter and enhanced green fluorescent protein (GFP) with or without Pax6, i.e., Ad-GFP-Pax6 (experimental) and Ad-GFP (control) (Fig. 4A) to be at the multiplicity of infection (MOI) of 100 (Fig. 4B, *p < 0.1 and **p < 0.05, n = 3).